size exclusion chromatography sec buffer Search Results


size exclusion chromatography purification buffer  (Anatrace)
90
Anatrace size exclusion chromatography purification buffer
Size Exclusion Chromatography Purification Buffer, supplied by Anatrace, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/size exclusion chromatography purification buffer/product/Anatrace
Average 90 stars, based on 1 article reviews
size exclusion chromatography purification buffer - by Bioz Stars, 2026-04
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size-exclusion chromatography-multi-angle light scattering buffer  (Fisher Scientific)
90
Fisher Scientific size-exclusion chromatography-multi-angle light scattering buffer
Pot1-DBD forms primarily a 1:1 complex at low concentrations and can form a larger complex at high concentrations in the presence of 12mer, but not 15mer. Amino acid mutation or base substitution can switch between these two binding modes. ( A ) RI traces (lines) and molar masses calculated by MALS (markers) of 34 μM Pot1-DBD+15mer and 34 μM Pot1-DBD+12mer complexes in <t>20</t> <t>mM</t> Tris–HCl pH 8.0, 50 mM NaCl. ( B ) Increasing the concentration of NaCl to 400 mM causes Pot1-DBD+12mer to elute earlier than Pot1-DBD+15mer and with a variable but larger average molar mass. Protein and DNA are both at 34 μM. ( C ) Increasing the concentration of Pot1-DBD to 170 μM and increasing the molar ratio to 2:1, Pot1-DBD:oligonucleotide pushes the 12mer-binding mode toward the 2:1 complex. This 12mer-binding mode can be forced to form a 1:1 complex upon 12mer_T9A substitution. Conversely, Pot1-DBD_Y224A mutation or 15mer_G13C substitution can switch the 15mer-binding mode to the 12mer-binding mode. All complexes were injected at 170 μM protein + 85 μM DNA.
Size Exclusion Chromatography Multi Angle Light Scattering Buffer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/size-exclusion chromatography-multi-angle light scattering buffer/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
size-exclusion chromatography-multi-angle light scattering buffer - by Bioz Stars, 2026-04
90/100 stars
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buffer for size exclusion chromatography  (CANTEST Ltd)
90
CANTEST Ltd buffer for size exclusion chromatography
Pot1-DBD forms primarily a 1:1 complex at low concentrations and can form a larger complex at high concentrations in the presence of 12mer, but not 15mer. Amino acid mutation or base substitution can switch between these two binding modes. ( A ) RI traces (lines) and molar masses calculated by MALS (markers) of 34 μM Pot1-DBD+15mer and 34 μM Pot1-DBD+12mer complexes in <t>20</t> <t>mM</t> Tris–HCl pH 8.0, 50 mM NaCl. ( B ) Increasing the concentration of NaCl to 400 mM causes Pot1-DBD+12mer to elute earlier than Pot1-DBD+15mer and with a variable but larger average molar mass. Protein and DNA are both at 34 μM. ( C ) Increasing the concentration of Pot1-DBD to 170 μM and increasing the molar ratio to 2:1, Pot1-DBD:oligonucleotide pushes the 12mer-binding mode toward the 2:1 complex. This 12mer-binding mode can be forced to form a 1:1 complex upon 12mer_T9A substitution. Conversely, Pot1-DBD_Y224A mutation or 15mer_G13C substitution can switch the 15mer-binding mode to the 12mer-binding mode. All complexes were injected at 170 μM protein + 85 μM DNA.
Buffer For Size Exclusion Chromatography, supplied by CANTEST Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer for size exclusion chromatography/product/CANTEST Ltd
Average 90 stars, based on 1 article reviews
buffer for size exclusion chromatography - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

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Pot1-DBD forms primarily a 1:1 complex at low concentrations and can form a larger complex at high concentrations in the presence of 12mer, but not 15mer. Amino acid mutation or base substitution can switch between these two binding modes. ( A ) RI traces (lines) and molar masses calculated by MALS (markers) of 34 μM Pot1-DBD+15mer and 34 μM Pot1-DBD+12mer complexes in 20 mM Tris–HCl pH 8.0, 50 mM NaCl. ( B ) Increasing the concentration of NaCl to 400 mM causes Pot1-DBD+12mer to elute earlier than Pot1-DBD+15mer and with a variable but larger average molar mass. Protein and DNA are both at 34 μM. ( C ) Increasing the concentration of Pot1-DBD to 170 μM and increasing the molar ratio to 2:1, Pot1-DBD:oligonucleotide pushes the 12mer-binding mode toward the 2:1 complex. This 12mer-binding mode can be forced to form a 1:1 complex upon 12mer_T9A substitution. Conversely, Pot1-DBD_Y224A mutation or 15mer_G13C substitution can switch the 15mer-binding mode to the 12mer-binding mode. All complexes were injected at 170 μM protein + 85 μM DNA.

Journal: Nucleic Acids Research

Article Title: The telomeric protein Pot1 from Schizosaccharomyces pombe binds ssDNA in two modes with differing 3′ end availability

doi: 10.1093/nar/gku680

Figure Lengend Snippet: Pot1-DBD forms primarily a 1:1 complex at low concentrations and can form a larger complex at high concentrations in the presence of 12mer, but not 15mer. Amino acid mutation or base substitution can switch between these two binding modes. ( A ) RI traces (lines) and molar masses calculated by MALS (markers) of 34 μM Pot1-DBD+15mer and 34 μM Pot1-DBD+12mer complexes in 20 mM Tris–HCl pH 8.0, 50 mM NaCl. ( B ) Increasing the concentration of NaCl to 400 mM causes Pot1-DBD+12mer to elute earlier than Pot1-DBD+15mer and with a variable but larger average molar mass. Protein and DNA are both at 34 μM. ( C ) Increasing the concentration of Pot1-DBD to 170 μM and increasing the molar ratio to 2:1, Pot1-DBD:oligonucleotide pushes the 12mer-binding mode toward the 2:1 complex. This 12mer-binding mode can be forced to form a 1:1 complex upon 12mer_T9A substitution. Conversely, Pot1-DBD_Y224A mutation or 15mer_G13C substitution can switch the 15mer-binding mode to the 12mer-binding mode. All complexes were injected at 170 μM protein + 85 μM DNA.

Article Snippet: Pot1-DBD and the Y224A mutant were buffer exchanged and concentrated in size-exclusion chromatography-multi-angle light scattering (SEC-MALS) buffer (20 mM Tris–HCl pH 8.0 and 50/400 mM NaCl) using 10 000 MWCO Vivaspin Turbo concentrators pre-rinsed with water (Fisher Scientific).

Techniques: Mutagenesis, Binding Assay, Concentration Assay, Injection